Fig. 2. Association of PDIA6 is promoted by its C-terminal KDEL sequence. (A) PDIA6 lacking its KDEL sequence shows a reduced cell surface association. PDIA6 without its KDEL sequence (PDIA6wo) and complete PDIA6 were both fluorescently labeled and, after incubation and washes, analyzed by flow cytometry. The p-value for PDIA6 compared with that for PDIA6 without its KDEL sequence (PDIA6wo) was calculated, and as **p<0.01, was considered as significant. (B) Representative graph from the three independent experiments summarized in the bar diagram shown in Fig. 2A. All experiments were performed at least in triplicate. (C) Bar diagram summarizing the results of flow cytometric analysis of HEK293T cells stained with 0.5 µM fluorescently labeled PDIA6. This interaction can be either reduced by addition of unlabeled KDEL peptide or unlabeled PDIA6. Fluorescently labeled IL-6 was used as a negative control, since HEK293T cells do not express IL-6R. P-values were calculated and are labeled regarding their significance with *p< 0.05, **p<0.01, ***p<0.001. (D) Representative graph of the experiment summarized in Fig. 2C.